Sysmic

Sysmic Annual Meeting

Sysmic will hold its annual meeting in October, 2021 in Flemingsberg.

New standards to report cell migration studies

Strömblad participated in an international effort to develop reporting standards for the cell migration field, summarized in GigaScience.

Masuzzo, P., Gonzalez-Beltran, A., Ampe, C., Besson, S., Eibl, R., Friedl, P., Gunzer, M., Kittisopikul, M., Le Dévédec, S., Leo, S., Moore, J., Prilusky, J., Rocca-Serra, P., Roudot, P., Sansone, S-A., Sergeant G., Strömblad, S., Swedlow, J.R., Van Troys, M., Zaritsky, A., Martens, L. Community Standards for Open Cell Migration Data., GigaScience (2020).

Improved segmentation of patches in microscopy

The Wählby lab reported on their new method to improve segmentation of patches in microscopic images:

Pielawski N and Wählby C. Introducing Hann windows for reducing edge-effects in patch-based image segmentation. https://arxiv.org/abs/1910.07831. Accepted for publication in PlosONE (2020).

A simplified approach to traction force microscopy through deep learning

The Wählby and Strömblad labs presented their work to simplify the analysis of traction force microscopy:

Pielawski N, Hu J, Strömblad S, Wählby C. In Silico Prediction of Cell Traction Forces. arXiv preprint arXiv:1910.07380. 2019. DOI: arXiv:1910.07380, accepted for publication in The proceedings of IEEE ISBI 2020.

Diagnosing breast cancer with spatial transcriptomics

The Daub lab reported on their progress in spatial transcriptomics for breast cancer diagnosis.

Yoosuf N, Navarro JF, Salmén F, Ståhl PL, Daub CO. Identification and transfer of spatial transcriptomics signatures for cancer diagnosis. Breast Cancer Res 22,16 (2020).

Sysmic Annual Meeting

Sysmic will hold its annual meeting on January 21, 2020 in Flemingsberg.

Review of deep learning in microscopy image analysis

The Wählby group reviewed the use of deep learning in image analysis.

Gupta, A., Harrison, P.J., Wieslander, H., Pielawski, N., Kartasalo, K., Partel, G., Solorzano, L., Suveer, A., Klemm, A.H., Spjuth, O. and Sintorn, I.M., and Wählby, C. Deep Learning in Image Cytometry: A Review. Cytometry A 95(4), 366-380 (2019).

Sysmic Annual Meeting

Sysmic will hold its annual meeting January 9-10, 2019 in Uppsala. Registration should be made to Staffan Strömblad before December 18.

Developments in systems microscopy to understand cell migration

Strömblad & Lock summarized their developments of the systems microscopy approach to study cell migration. 

Strömblad S. & Lock J.G. Using Systems Microscopy to Understand the Emergence of Cell Migration from Cell Organization. Meth Mol Biol, vol 1749. (2018).

Outlook into the future of protein diagnostics

The Landergen group outlaid the future of molecular based diagnostics.

Landegren U, Al-Amin RA, Björkesten J., A myopic perspective on the future of protein diagnostics., N Biotechnol.45, 14-18 (2018).

Improvemed proximity ligation detection method

The Landegren lab reported in two papers on their improved proximity ligation detection method:

Klaesson A, Grannas K, Ebai T, Heldin J, Koos B, Raykova D, Oelrich J, Arngården L, Söderberg O, Landegren U. Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes. Sci Rep 8, 5400 (2018).

Zieba A, Ponten F, Uhlén M, Landegren U., In situ protein detection with enhanced specificity using DNA-conjugated antibodies and proximity ligation. Mod Pathol. 31, 253 (2018).

Sysmic researchers have identified mechanism for amoeboid cell migration

Xiaowei Gong and his colleagues in the Strömblad laboratory identified a role for RhoB in cell mebrane blebbing and amoeboid cell migration in a recently published study. The role of RhoB is linked to its subcellular localization where RhoB can be recycled to the plasma membrane and there serve to promote blebbing and thereby promote cell migration. This recycling occurs through Rab11-positive vesicles and is dependent on KIF13A.

Publication: Gong X, Didan Y, Lock JG, Strömblad S. KIF13A-regulated RhoB plasma membrane localization governs membrane blebbing and blebby amoeboid cell migration. EMBO J. 2018 Sep 3;37(17). pii: e98994. doi: 10.15252/embj.201898994. Epub 2018 Jul 26.

New cell adhesion structure discovered

John Lock and his colleagues in the Strömblad laboratory, in collaboration with researchers in Manchester, UK, has discovered a novel cellular structure in the form of Reticular adhesions, an cell-matrix adhesion structure with a unique componentry. The researchers also found that the Reticular adhesions mediate cell attachment during mitotic round-up, when all previously know adhesion structures disassemble. Given the central role of cell-matrix adhesion in mesenchymal cell migration, the Reticular adhesions may also affect cell migration, but this remains to be examined.

Publication:  Lock JG, Jones MC, Askari JA, Gong X, Oddone A, Olofsson H, Göransson S, Lakadamyali M, Humphries MJ, Strömblad S. Reticular adhesions are a distinct class of cell-matrix adhesions that mediate attachment during mitosis. Nat Cell Biol. 2018 Nov;20(11):1290-1302. doi: 10.1038/s41556-018-0220-2. Epub 2018 Oct 22.

The nanoscale organization of cell-matrix contact receptors unraveled

Cell-matrix contact points such as focal adhesions are critical for cell migration. In a study published in The Journal of Cell Biology, Staffan Strömblad and colleagues used superresolution microscopy to, for the first time, characterize the nanoscale organization of integrin cell-matrix receptors within focal adhesions. Surprisingly, they found that active (matrix-bound) and inactive (unbound) 1-integrins segregate into distinct nanoclusters within the focal adhesions, suggesting the existence of a novel mechanism for collective integrin activity regulation